CME Course

 

“Podocytes in vitro”
lecture- and practical course

 

 

 

June 7-9th, 2007
National Institute for Cell Biology
Dublin City University
Ireland

 

 

 

 

 

ERA-EDTA
European Council for Accreditation in CME in Nephrology, Dialysis and Transplantation (ECANDT of the ERA-EDTA accredited by the European Accreditation Council for CME - EACCME).
Chair-person:
R. Coppo (Italy)
Secretary:
L.M. De Francisco (Spain)
Members:
J. Floege (Germany)
R. Vanholder (Belgium)
A. Wiecek (Poland)
C. Zoccali (Italy)
J. Cannata-Andia, ERA-EDTA President (Spain)

 

CME Courses Coordinators:

Basic Science and Clinical Nephrology:
R. Coppo (Italy) and P. Ronco (France).
Chronic Renal Failure, Hypertension and Clinical Epidemiology:
C. Zoccali (Italy) and G. London (France).
Dialysis:
R. Vanholder (Belgium) S. Davies (UK) and C. Wanner (Germany).
Transplantation:
C. Ponticelli (Italy), A. Torres (Spain) and K. Olgaard (Denmark).
Collaboration with ISN for courses in Eastern Europe:
N. Lameire (Belgium), A. Covic (Romania).

 

Local Coordinator: Harry Holthofer harry.holthofer@dcu.ie

 

For information www.cbas.ie/news.html

 

SCIENTIFIC PROGRAMME

 

DAY 1 (afternoon 7th of June)
     
    
Harry Holthofer
Welcome and Introduction
     
Podocytes in vivo and in vitro: do we understand them well enough?
Harry Holthofer, Dublin City University, Ireland
   
Podocytes in culture: past, present and future
Peter Mundel, Mount Sinai School of Medicine, New York
    
Studying podocyte proteins in vitro using mammalian overexpression cell culture models
Sanna Lehtonen, University of Helsinki, Finland
   
Coffee
    
Human podocytes - advantages, difficulties and future perspectives
Moin Saleem, University of Bristol, UK
    
Establishment and characterization of rat podocyte cell line
Hidetake Kurihara, Juntendo University School of Medicine, Tokyo
    

Pathophysiologic studies of podocytes in vitro
Karlhans Endlich, University of Greifswald, Germany
   
Dinner
    
    
DAY 2 (8th of June)
     
Culture of mammalian cells for LifeScience research and bioindustry utilisation
Martin Clynes, Dublin City University
   
Primary podocyte cell cultures
MariaPia Rastaldi, Fondazione DÀmico, Milano, Italy
   
Glucocorticoid Action in Podocytes
Richard Ransom, University of Michigan, AnnArbor
   
LUNCH
    
Laboratory experimentation
   
   
DAY 3 (9th of June)
    
Laboratory experimentation continues
   
Wrap-up and adjourn
   

LUNCH
     
   

Programme of the practical course (in groups of five plus a tutor):
   
1) Microscopy/familiarisation to the different podocyte cell lines
- differentiated and non-differentiated cells, comparisons between different lines
    
2) Immunofluorescence microscopy demonstrations
- defining the minimal essential set of markers to verify podocytic nature
-reviewing other used criteria for identification
  

3) Primary podocytic cells:
-how to create, maintain and use
   
4) Meet the expert:
-possibility for in-depth discussions of problems and issues with the ones who created the cell lines
    
5) Independent group activation to define the differences between cell lines, based on experiences at microscopy, meeting with experts, on the basis of the material delivered to attendants
   
6) Tour around modern cell biologic facilities of NICB as a basis for lab-planning, resourcing, collaborations.