EUROPEAN TRAINING CENTRES

El Prado - Madrid

Centro de Investigaciones Biologicas. (Consejo Superior de Investigaciones Cientificas)
Madrid, Spain

 

HEAD OF THE UNIT Prof Santiago Lamas Peláez
   
  Molecular_Pathophisiology of the vascular wall

RESEARCH AND LABORATORY

Fernando Rodríguez Pascual, Antonio Martínez Ruiz, María Estrella Soria Lopez and Mariano Redondo Horcajo, Cristina de Alvaro y Pérez, Cecilia Gonzalez de Orduña Martín, Maria Angeles Higueras López, Noemí Magan Marchal.

 

CONTACT INFORMATION
E-MAIL slamas@cib.csic.es
PHONE: +34 918373112 (Ext 4302)
FAX: +34 915360432
FULL POSTAL ADDRESS Ramiro de Maeztu, 9, 28040 Madrid
WEBSITE

http://www.cib.csic.es/en/grupo.php?idgrupo=37

 

 

RESEARCH INTERESTS

BASIC RESEARCH

1. Regulation of genes involved in vascular pathology. Role of nitric oxide and redox state.
2. Extracellular matrix remodelation by nitric oxide. Animal hypertension models for the analysis of metalloproteases expression and activity in atherosclerosis.
3. Role of nitric oxide as regulator of genes involved in inflammatory and proliferative processes in vascular wall cells. Subproject: Role of nitric oxide as modulator of vascular wall cellular response to pro-inflammatory agents.
4. Molecular mechanisms of vascular damage: a proteomic approach and development of animal models.
5. Role of TGF-beta/ET-1pathway as a target in prevention of atherosclerosis. Development of experimental models.
6. Animal models for the study of the TGF-beta/ET-1pathway in cardiovascular diseases.
7. Thematic network in cardiovascular diseases “RECAVA”.
8. Molecular targets in nitrosative endothelial damage produced by calcineurin inhibitors.
9. Post-translational modifications induced by oxidative and nitrosative stress in proteins from vascular endothelium: proteomical identification and functional evaluation.
10. Risk evolution and treatment factors of the cardiovascular diseases and their  molecular and cellular mechanism.

 

Peer Reviewed Publications in International Journals (only English) 2000-2006

Publications List:

Transcriptional induction of endothelial nitric oxide gene (eNOS) by Cyclosporine A: a role for AP-1.
J. Navarro-Antolín, J. Rey-Campos, S. Lamas.
Journal of Biological Chemistry/ 275: 3075-3080, 2000.
   
Novel application of S-nitrosoglutathione-Sepharose to identify proteins which are potential targets for S-nitrosoglutathione-induced mixed-disulphide formation.
Peter Klatt, Estela Pineda-Molina, Dolores Pérez-Sala, S.Lamas.
Biochemical Journal/ 349: 567-578, 2000.
   
Involvement of Rho GTPases in the Transcriptional Inhibition of Preproendothelin-1 Gene Expression by Simvastatin in Vascular Endothelial Cells.
O. Hernández-Perera, D. Pérez-Sala, E. Soria, S.Lamas.
Circulation Research / 87: 616-622, 2000.
   
Imbalance in endothelial vasoactive factors as a possible cause of cyclosporin toxicity: A role for endothelin-converting enzyme.
L. González-Santiago, S.López-Ongil, S.Lamas, C. Quereda, M. Rodríguez-Puyol, D.Rodríguez-Puyol.
Journal Laboratory Clinical Medicin/e 136 (5): 395-401, 2000.
   
Posttranscriptional regulation of the human inducible nitric oxide synthase by nitric oxide- cyclic GMP pathway.
D. Pérez-Sala, E. Cernuda, M. Díaz-Cazorla, F. Rodríguez-Pascual, S. Lamas.
American Journal of Physiology /280, F466-F473, 2001.
   
Nitrosative stress by Cyclosporine-A in the endothelium: studies with the NO-sensitive probe Diaminofluorescein -2/diacetate using flow cytometry.
Javier Navarro-Antolin, Santiago Lamas.
Nephrology, Dialysis and Transplantation,/ 16 (Supp. 6): 1-4, 2001.
   
Formation of peroxynitrite in vascular endothelial cells exposed to Cyclosporine A.
Javier Navarro-Antolín, Maria José López-Muñoz, Peter Klatt, Javier Soria, Thomas Michel, Santiago Lamas.
FASEB Journal /15: 1291-1293, 2001.
   
Formation of peroxynitrite in vascular endothelial cells exposed to Cyclosporine A.
Javier Navarro-Antolin, María José López-Muñoz, Peter Klatt, Javier Soria, Thomas Michel, Santiago Lamas.
FASEB J / 10.1096/ fj.00-0636 fje, /2001
   
Cooperation between Low Density Lipoproteins and IGF-1 in the Promotion of Mitogenesis in Vascular Smooth Muscle Cells.
Belén González, Santiago Lamas, Elvira M. Melián.
Endocrinology/ 142: 4852-4860, 2001.
   
Glutathionylation of the p50 subunit of NF-**?**B: a mechanism for redox -induced inhibition of DNA binding.
Estela Pineda-Molina, Peter Klatt, Jesús Vázquez, Anabel Marina, Mario Garcia de Lacoba, Dolores Pérez-Sala, Santiago Lamas.
Biochemistry/ 40: 14134-14142, 2001.
    
Nitric oxide as a regulator of gene expression: Studies with the transcription factor proteins cJun and p50.
Estela Pineda-Molina, Santiago Lamas.
Biofactors/* *15 (2-4): 113-5, 2001.
   
Nitric oxide regulates matrix metalloprotease-13 expression and activity in endothelium.
Carlos Zaragoza, Milagros Balbín, Carlos López-Otín, Santiago Lamas.
Kidney International/ 61: 804-808, 2002.
   
Superoxide limits cyclosporine-A-induced formation of peroxynitrite in endothelial cells.
Javier Navarro-Antolín, María José López-Muñoz, Javier Soria, Santiago Lamas.
Free Radical Biology & Medicine/ 32: 702-711, 2002.
   
Expression of endoglin in human mesangial cells: modulation of extracellular matrix synthesis.
L Díez Marques, R Ortega-Velázquez, C Langa, A Rodríguez-Barbero, JM López-Novoa, Santiago Lamas, C Bernabeu.
Biochim Biophys Acta/ 1587(1): 36-44, 2002.
   
PPAR agonists amplify iNOS expression while inhibiting NF-**?**B: Implications for mesangial cell activation by cytokines.
Eva Cernuda-Morollón, Fernando Rodríguez-Pascual, Peter Klatt, Santiago Lamas, Dolores Pérez-Sala.
J Am Soc Neprol/ 13(9): 2223-31, 2002.
   
Activation of the MAP Kinase ERK 1,2 by the NO-cGMP-PKG Axis Regulates the Expression of Matrix Metalloproteinase-13 in Vascular Endothelial Cells.
Carlos Zaragoza, Estrella Soria, Esther López, Darren Browning, Milagros Balbín, Carlos López-Otín, Santiago Lamas.
Molecular Pharmacology/ 62(4): 927-35, 2002
   
Functional cooperation between Smad proteins and AP-1 regulates transforming growth factor- β-mediated induction of endothelin-1 expression.
Fernando Rodríguez-Pascual, Mariano Redondo-Horcajo, S. Lamas.
Circulation research 92: 1288-95: 2003.
   
Detection and proteomic identification of S nitrosylated proteins in endothelial cells.
Martínez-Ruiz A, Lamas S.
Archives of Biochemistry and Biophysics 423: 192-199, 2004.
   
Transforming Growth Factor-B Induces Endothelin-1 Expression Through Activation of the Smad Signaling Pathway.
Fernando Rodríguez-Pascual, Francisco Manuel Reimunde, Mariano Redondo-Horcajo, Santiago Lamas.
Journal of Cardiovascular  Pharmacology 44: S39 – S42, 2004.
   
Matrix metalloproteinase 13 mediates nitric oxide activation of endothelial cell migration.
López-Rivera E, Lizarbe T, Martínez-Moreno M, López-Novoa JM, Rodríguez-Barbero A, Rodrigo J, Fernández AP, Alvarez-Barrientos A, Lamas S, Zaragoza C.
Proceedings of the National Academy of Sciences USA. 102: p. 3685-3690, 2005.
   
Endothelin-1 expression is strongly repressed by AU-rich elements in the 3' untranslated region o the gene.
Reimunde FM, Castanares C, Redondo-Horcajo M, Lamas S, Rodriguez-Pascual F.
Biochemical Journal;   387 p:763-772. . 2005.
   
PGC-1 alpha regulates the mitochondrial antioxidant defense system in vascular endothelial cells.
Valle I, Alvarez-Barrientos A, Arza E, Lamas S, Monsalve M.
Cardiovascular Research. 2005 Jun 1;66(3):562-573.
   
S-Nitrosylation of Hsp90 promotes the inhibition of its ATPase and eNOS regulatory activities.
Martínez-Ruiz A, Villanueva L, González de Orduña C, López-Ferrer D, Higueras M.A, Tarín C, Rodríguez-Crespo I, Vázquez J, Lamas S.
Proceedings of the National Academy of Sciences USA (2005):8525-8530
    
Cbfa-1-mediated expression of MMP-13 in osteoblasts is mediated by nitric oxide.
Zaragoza C, López-Rivera E, García-Rama C, Saura M, Martínez-Ruiz A, Lizarbe T.R, Martín-de-Lara F, Lamas S,
Journal of Cell Science, May 1;119(Pt 9):1896- 902.
   
Constitutive ALK5-independent JNK activation contributes to endothelin -1 over-expression in pulmonary fibrosis: Evidence of an autocrine endothelin loop operating through the endothelin A and B receptors.
Shi-wen X, Rodriguez-Pascual F, Lamas S, Holmes A, Howat S, Pearson JD, Dashwood MR, Du Bois RM, Denton CP, Black CM, Abraham DJ, Leask A.
Molecular and Cellular Biology, 2006  26(14):5518-552.
   
Nitric oxide regulates mitochondrial oxidative stress protection via the transcriptional coactivator PGC-1alpha
Borniquel S, Valle I, Cadenas S, Lamas S, Monsalve M.
FASEB J. 2006 Sep;20(11):1889-91. Epub 2006 Aug 4

 

European Fellows programme

Specify if the Unit accepts fellows from European countries for:
 
   
Short (1-2 month) well focused training programs either clinically or laboratory oriented: Yes
Long (at least 1 year) research fellowships: Yes
Specify corresponding availability of grants: No
Specify if the Unit may provide help for accommodation: Yes

 

Prerequisites for Fellowship

Language (specify) : English.

Work/Research experience (specify) :Bench experience.

Other prerequisites (specify): Letters of recommendation.